Rao V L, Butterworth R F
Neuroscience Research Unit, Hôpital Saint-Luc (University of Montreal), Quebec, Canada.
Alcohol Clin Exp Res. 1995 Apr;19(2):523-6. doi: 10.1111/j.1530-0277.1995.tb01541.x.
Activities of thiamine monophosphatase (TMPase) and thiamine diphosphatase (TDPase) were measured in homogenates of brain tissue obtained at autopsy from eight alcoholic cirrhotic patients who died in hepatic coma and nine controls matched for age and for postmortem delay interval and free from neurological or psychiatric disorders, hepatic disease, or other conditions of grossly impaired nutritional status. Enzyme activities were measured by standard spectrophotometric techniques. Both TMPase and TDPase were distributed unevenly in brain with highest activities being recorded in temporal cortex. Regional correlations between TMPase and TDPase, however, were poor. TDPase activities in brain tissue from alcoholic cirrhotic patients were significantly increased in 5 of 6 brain regions, by 26 to 153% (p < 0.05). TMPase activities in alcoholic cirrhotics, on the other hand, were unchanged in all brain regions, with the exception of caudate nucleus where they were increased by 70% (p < 0.05). These findings add to the substantial body of evidence suggesting that alcoholic liver disease is associated with abnormal thiamine status and with altered thiamine neurochemistry. Increased TDP degradation resulting from increased activities of TDPase could contribute to the pathophysiological mechanisms involved in alcohol-related brain dysfunction.
对八名死于肝昏迷的酒精性肝硬化患者尸检获得的脑组织匀浆以及九名年龄、死后延迟时间相匹配且无神经或精神疾病、肝脏疾病或其他严重营养状况受损情况的对照者的脑组织匀浆，测定了硫胺素单磷酸酶（TMPase）和硫胺素二磷酸酶（TDPase）的活性。酶活性通过标准分光光度技术进行测定。TMPase和TDPase在脑中分布不均，颞叶皮质的活性最高。然而，TMPase和TDPase之间的区域相关性较差。酒精性肝硬化患者脑组织中TDPase的活性在6个脑区中的5个显著增加，增幅为26%至153%（p<0.05）。另一方面，酒精性肝硬化患者脑中TMPase的活性在所有脑区均未改变，但尾状核除外，其活性增加了70%（p<0.05）。这些发现进一步充实了大量证据，表明酒精性肝病与硫胺素状态异常以及硫胺素神经化学改变有关。TDPase活性增加导致的TDP降解增加可能有助于酒精相关脑功能障碍的病理生理机制。
