一种与人类巨细胞病毒基因UL36和UL37的内含子-外显子边界互补的反义寡核苷酸具有强大的抗病毒活性。
Potent antiviral activity of an antisense oligonucleotide complementary to the intron-exon boundary of human cytomegalovirus genes UL36 and UL37.
作者信息
Pari G S, Field A K, Smith J A
机构信息
Hybridon Inc., Worcester, Massachusetts 01605, USA.
出版信息
Antimicrob Agents Chemother. 1995 May;39(5):1157-61. doi: 10.1128/AAC.39.5.1157.
Abstract
An antisense phosphorothioate oligonucleotide complementary to the intron-exon boundary of human cytomegalovirus genes UL36 and UL37 (UL36ANTI) reduced the yield of infectious virus by 99% and inhibited human cytomegalovirus DNA replication at a concentration of 0.08 microM. In addition, oligonucleotides with base substitutions which resulted in base pair mismatches showed lesser degrees of activity, indicating a sequence-specific antisense mechanism. UL36ANTI was also shown to inhibit DNA replication of ganciclovir-resistant strains and human cytomegalovirus clinical isolates.
摘要
一种与人类巨细胞病毒基因UL36和UL37的内含子-外显子边界互补的硫代磷酸反义寡核苷酸（UL36ANTI），在浓度为0.08微摩尔时可使感染性病毒产量降低99%，并抑制人类巨细胞病毒DNA复制。此外，导致碱基对错配的碱基取代寡核苷酸表现出较低程度的活性，表明存在序列特异性反义机制。UL36ANTI还被证明可抑制更昔洛韦耐药菌株和人类巨细胞病毒临床分离株的DNA复制。
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