Ariyoshi N, Oguri K, Koga N, Yoshimura H, Funae Y
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Biochem Biophys Res Commun. 1995 Jul 17;212(2):455-60. doi: 10.1006/bbrc.1995.1991.
Metabolism of 2,4,5,2',4',5'-hexachlorobiphenyl was studied with cDNA-expressed human P450 2B isoform, CYP2B6. 3-Hydroxy-2,4,5,2',4',5'-hexachlorobiphenyl was identified as a major metabolite, and the formation activity was compared with that of dog CYP2B11 and guinea pig P450GP-1. The activity of 3-hydroxylation was comparable with that of P450GP-1, but one-tenth of CYP2B11. These results indicate that P450 2B in humans as well as other animal species can metabolize 2,4,5,2',4',5'-hexachlorobiphenyl, and the reason why this PCB congener remained most abundantly in human bodies is discussed.
利用cDNA表达的人P450 2B同工型CYP2B6研究了2,4,5,2',4',5'-六氯联苯的代谢。3-羟基-2,4,5,2',4',5'-六氯联苯被鉴定为主要代谢产物，并将其生成活性与犬CYP2B11和豚鼠P450GP-1的活性进行了比较。3-羟基化活性与P450GP-1相当，但仅为CYP2B11的十分之一。这些结果表明，人类以及其他动物物种中的P450 2B均可代谢2,4,5,2',4',5'-六氯联苯，并对该多氯联苯同系物在人体中残留量最高的原因进行了讨论。
