Laredo J, Hamilton B P, Hamlyn J M
Department of Physiology, University of Maryland School of Medicine, Baltimore 21201, USA.
Biochem Biophys Res Commun. 1995 Jul 17;212(2):487-93. doi: 10.1006/bbrc.1995.1996.
Recent experiments have demonstrated the secretion of a ouabain-like compound from primary cultures of bovine adrenocortical cells (1). To determine the relative contribution of the outer zones of the adrenal cortex to the secretion of endogenous ouabain, we measured the content of this steroid in zona glomerulosa and zona fasciculata tissue freshly excised from bovine adrenals and in the secretion media of primary cell cultures derived from these zones. The tissue content of endogenous ouabain was 5.7-fold higher in the zona glomerulosa than in the zona fasciculata. The concentration of endogenous ouabain was 4.1-fold higher in 24-hour conditioned media from zona glomerulosa than in zona fasciculata cultures. Angiotensin II and ACTH stimulated the secretion of endogenous ouabain from zona glomerulosa cultures, but had no effect on zona fasciculata cultures. These findings suggest that endogenous ouabain is primarily synthesized and secreted from the adrenal zona glomerulosa.
最近的实验已证明牛肾上腺皮质细胞原代培养物会分泌一种哇巴因样化合物(1)。为了确定肾上腺皮质外层区域对内源性哇巴因分泌的相对贡献，我们测量了从牛肾上腺新鲜切下的球状带和束状带组织以及源自这些区域细胞原代培养物的分泌培养基中这种类固醇的含量。内源性哇巴因的组织含量在球状带比束状带高5.7倍。来自球状带的24小时条件培养基中内源性哇巴因的浓度比束状带培养物中的高4.1倍。血管紧张素II和促肾上腺皮质激素刺激球状带培养物中内源性哇巴因的分泌，但对束状带培养物没有影响。这些发现表明内源性哇巴因主要由肾上腺球状带合成和分泌。
