Wang Z, Lange A B, Orchard I
Department of Zoology, University of Toronto, Ontario, Canada.
Biochem Biophys Res Commun. 1995 Jul 17;212(2):531-8. doi: 10.1006/bbrc.1995.2002.
A series of studies, which have investigated the membrane receptor-mediated signal transduction of PDVDHVFLRFamide in the locust oviduct, suggest the presence of a novel ligand-receptor reaction system. In this system, inhibitory peptides and stimulatory peptides share a single receptor by having the same binding sequence, VFLRFamide, but are able to produce opposite muscle responses due to the differences in activation sites. In this research, the results from both "guanine nucleotide effect assay" and GTPase assay indicated that the receptors for the inhibitory and stimulatory FMRFamide-related peptides (FaRPs) are coupled with G proteins and both inhibitory and excitatory effects of FaRPs on locust oviduct are through the activation of G proteins. Thus it is very likely that the receptor is coupled with two different G proteins. The activation of one is responsible for the inhibitory effect and the activation of the other is responsible for the stimulatory effect.
一系列针对蝗虫输卵管中PDVDHVFLRFamide的膜受体介导信号转导的研究表明，存在一种新型的配体-受体反应系统。在这个系统中，抑制性肽和刺激性肽通过具有相同的结合序列VFLRFamide共享一个受体，但由于激活位点的差异，它们能够产生相反的肌肉反应。在本研究中，“鸟嘌呤核苷酸效应测定”和GTP酶测定的结果均表明，抑制性和刺激性FMRF酰胺相关肽（FaRPs）的受体与G蛋白偶联，并且FaRPs对蝗虫输卵管的抑制和兴奋作用均通过G蛋白的激活来实现。因此，该受体很可能与两种不同的G蛋白偶联。其中一种的激活负责抑制作用，另一种的激活负责刺激作用。
