从蝗虫腹神经索中分离、测序及鉴定FMRF酰胺相关肽的生物活性

Isolation, sequence, and bioactivity of FMRFamide-related peptides from the locust ventral nerve cord.

作者信息

Lange A B, Peeff N M, Orchard I

机构信息

Department of Zoology, University of Toronto, Ontario, Canada.

出版信息

Peptides. 1994;15(6):1089-94. doi: 10.1016/0196-9781(94)90075-2.

DOI:10.1016/0196-9781(94)90075-2

PMID:7991453

Abstract

The ventral nerve cord of the locust, Locusta migratoria, was examined for the presence of FMRFamide-related peptides (FaRPs). RP-HPLC coupled to an RIA specific for extended -RFamides revealed the presence of several FaRPs eluting at different percentages of acetonitrile. The sequences of five of these peptides were determined. Two sequences are identical to the two peptides previously sequenced from brain and retrocerebral complex of Locusta. These two peptides (PDVDHVFLRFamide and ADVGHVFLRFamide) were inhibitory when tested on locust oviduct contractions. The other peptides are novel with sequences of GQERNFLRFamide, AXXRNFIRFamide, and AFIRFamide. The synthesized peptides were stimulatory when tested on locust oviduct contractions, increasing the frequency and amplitude of spontaneous contractions and resulting in a basal contraction.

摘要

对飞蝗（Locusta migratoria）的腹神经索进行了检查，以确定是否存在FMRF酰胺相关肽（FaRPs）。反相高效液相色谱（RP-HPLC）与针对延伸-RFamides的放射免疫分析（RIA）联用，揭示了几种在不同乙腈百分比下洗脱的FaRPs的存在。确定了其中五种肽的序列。其中两个序列与先前从飞蝗的脑和后脑复合体中测序得到的两种肽相同。当对蝗虫输卵管收缩进行测试时，这两种肽（PDVDHVFLRFamide和ADVGHVFLRFamide）具有抑制作用。其他肽是新发现的，其序列分别为GQERNFLRFamide、AXXRNFIRFamide和AFIRFamide。当对蝗虫输卵管收缩进行测试时，合成的肽具有刺激作用，增加了自发收缩的频率和幅度，并导致基础收缩。