Salminen A, Liu P K, Hsu C Y
Division of Restorative Neurology, Baylor College of Medicine, Houston, TX 77030, USA.
Biochem Biophys Res Commun. 1995 Jul 26;212(3):939-44. doi: 10.1006/bbrc.1995.2060.
Transient focal ischemia-reperfusion in the cerebral cortex caused regional alteration of DNA-binding activities of transcription factors AP-1, CREB, Sp-1, and NF-kB. The changes were time-dependent. During the first 24 hr of reperfusion after 90 min ischemia, there was an increase in the binding activity of AP-1 only in the region surrounding the ischemic cortex. Five days after ischemia, an increase in the binding activities of CREB, Sp-1, and NF-kB, but not AP-1, was noted in the ischemic cortex, and to a lesser extent, Sp-1 and NF-kB, in the surrounding region. The binding activities of these transcription factors were reduced by hydrogen peroxide but could be restored by dithiothreitol and 2-mercaptoethanol. These results are the first demonstration of ischemia-induced differential regulation of transcription factor binding activities which are time-, region-, and redox state dependent.
大脑皮质的短暂局灶性缺血再灌注导致转录因子AP-1、CREB、Sp-1和NF-κB的DNA结合活性发生区域性改变。这些变化具有时间依赖性。在90分钟缺血后的再灌注最初24小时内，仅在缺血皮质周围区域AP-1的结合活性增加。缺血5天后，在缺血皮质中观察到CREB、Sp-1和NF-κB的结合活性增加，但AP-1未增加，在周围区域Sp-1和NF-κB的结合活性增加程度较小。这些转录因子的结合活性被过氧化氢降低，但可被二硫苏糖醇和2-巯基乙醇恢复。这些结果首次证明了缺血诱导的转录因子结合活性的差异调节，这种调节具有时间、区域和氧化还原状态依赖性。
