家兔心脏钠氢交换体（NHE-1）编码mRNA的发育变化

Developmental changes in mRNA encoding cardiac Na+/H+ exchanger (NHE-1) in rabbit.

作者信息

Chen F, Jarmakani J M, Van Dop C

机构信息

Division of Pediatric Cardiology, University of California, Los Angeles 90095-1752, USA.

出版信息

Biochem Biophys Res Commun. 1995 Jul 26;212(3):960-7. doi: 10.1006/bbrc.1995.2063.

DOI:10.1006/bbrc.1995.2063

PMID:7626137

Abstract

The faster recovery of cardiac contractility in newborn rabbit hearts during acute acidosis compared to adult hearts correlates with greater cellular activity of the Na+/H+ exchanger. We quantified mRNA encoding Na+/H+ exchanger-1 (NHE-1) in rabbit ventricles of fetal (27 days gestation), newborn (2-5 days), and adult (> 6 months) New Zealand white rabbits using reverse transcription-polymerase chain reaction (RT-PCR) and RNase protection assay, with GAPD mRNA as standard. Both RT-PCR and RNase protection assay revealed similar (p > 0.05) cardiac levels of NHE-1 mRNA in fetal and newborn hearts, while NHE-1 mRNA in these hearts was 1.7x and 1.6x greater than in adult hearts by both RT-PCR and RNase protection assay. These developmental changes in NHE-1 mRNA correlate with the developmental changes in Na+/H+ activity in sarcolemmal vesicles purified from rabbit heart.

摘要

与成年兔心脏相比，新生兔心脏在急性酸中毒期间心脏收缩力恢复更快，这与钠氢交换体的细胞活性更高有关。我们使用逆转录聚合酶链反应（RT-PCR）和核糖核酸酶保护试验，以甘油醛-3-磷酸脱氢酶（GAPD）mRNA作为标准，对胎龄27天的胎儿、出生2 - 5天的新生儿以及大于6个月的成年新西兰白兔心室中编码钠氢交换体-1（NHE-1）的mRNA进行了定量分析。RT-PCR和核糖核酸酶保护试验均显示，胎儿和新生兔心脏中NHE-1 mRNA的心脏水平相似（p > 0.05），而通过RT-PCR和核糖核酸酶保护试验，这些心脏中的NHE-1 mRNA比成年兔心脏中的分别高1.7倍和1.6倍。NHE-1 mRNA的这些发育变化与从兔心脏纯化的肌膜囊泡中钠氢活性的发育变化相关。