用酶法分析甜菜果胶的结构
Analysis of structure of sugar-beet pectin by enzymatic methods.
作者信息
Sakamoto T, Sakai T
机构信息
Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture, Japan.
出版信息
Phytochemistry. 1995 Jul;39(4):821-3. doi: 10.1016/0031-9422(95)00979-h.
The structure of alkali-soluble pectin (ASP) prepared from sugar-beet pulp was determined. ASP was sequentially degraded by pure galactanase, rhamnogalacturonase and arabinosidase and sugar compositions and NMR analysis of the products used to assign the following tentative structure to ASP: a backbone based on units of -->4)-alpha-D-GalpA-(1--> 2)-alpha-L-Rhap-(1-->, and side chains both of arabinan and of arabinogalactan.
测定了从甜菜粕制备的碱溶性果胶（ASP）的结构。用纯半乳聚糖酶、鼠李糖半乳糖醛酸酶和阿拉伯糖苷酶依次降解ASP，并对产物进行糖组成分析和核磁共振分析，从而为ASP确定了以下暂定结构：以→4)-α-D-半乳糖醛酸-(1→2)-α-L-鼠李糖-(1→单元为基础的主链，以及阿拉伯聚糖和阿拉伯半乳聚糖的侧链。
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