Hirose-Kumagai A, Oda-Tamai S, Akamatsu N
Department of Biochemistry, St. Marianna University School of Medicine, Kawasaki, Japan.
Biochem Mol Biol Int. 1995 Apr;35(4):881-8.
We examined the interaction of nucleoproteins with thyroid response element (TRE) by south-western method. The proteins that bound specifically to TRE were detected at apparent molecular weight about 30,000 and 15,000 - 10,000. After digestion of nucleoproteins blotted on PVDF membrane with Endoglycosidase H, the amounts of TRE that bound to the nucleoproteins were increased. These results show that carbohydrate moieties of nucleoproteins have an important role on the interaction of the nucleoproteins with TRE. T3 stimulated the binding of TRE to nucleoproteins. The amount of T3 binding to nucleoproteins was changed during liver regeneration. These results indicate that T3 changes the interaction of the nucleoproteins with TRE, so T3 serves as a transducing signal to modulate the gene regulating activity of TRE-binding nucleoproteins. Liver regeneration may be controlled by these modification of interaction of nucleoproteins with DNA.
我们通过蛋白质印迹法研究了核蛋白与甲状腺反应元件（TRE）的相互作用。在表观分子量约为30,000以及15,000 - 10,000处检测到与TRE特异性结合的蛋白质。用内切糖苷酶H消化印迹在聚偏二氟乙烯（PVDF）膜上的核蛋白后，与核蛋白结合的TRE量增加。这些结果表明，核蛋白的碳水化合物部分在核蛋白与TRE的相互作用中起重要作用。三碘甲状腺原氨酸（T3）刺激TRE与核蛋白的结合。在肝脏再生过程中，T3与核蛋白结合的量发生变化。这些结果表明，T3改变了核蛋白与TRE的相互作用，因此T3作为一种转导信号来调节与TRE结合的核蛋白的基因调控活性。肝脏再生可能受核蛋白与DNA相互作用的这些修饰的控制。
