Thiery J, Nebendahl K, Rapp K, Kluge R, Teupser D, Seidel D
Institute for Clinical Chemistry, University Hospital Grosshadern, Munich, FRG.
Arterioscler Thromb Vasc Biol. 1995 Aug;15(8):1181-8. doi: 10.1161/01.atv.15.8.1181.
In this report we describe the development of two rabbit strains, HAR (high atherosclerotic response) and LAR (low atherosclerotic response), and their propensities to develop atherosclerosis in the aorta despite similar levels of diet-induced hypercholesterolemia. Sixty-two randomly selected male New Zealand White rabbits were fed a cholesterol-enriched diet (0.5%) for 84 days and developed 57 +/- 25% sudanophilic lesions of the aortic surface; 12 rabbits showed a low atherosclerotic response (0% to 30% surface involvement), and 22 rabbits showed a high atherosclerotic response (70% to 100% surface involvement). The offspring of rabbits with low atherosclerotic response were used for breeding the strain of rabbits resistant to atherosclerosis (LAR strain), while the offspring of rabbits with high atherosclerotic response were used for breeding the HAR strain. Controlled breeding was started after the 4th generation and performed for the subsequent 6 generations. Thus, in the LAR rabbits the lipid-stainable surface area of aorta amounted to only 27 +/- 17% after 112 days of cholesterol feeding. On the other hand, in HAR rabbits, aortic surface involvement reached 85 +/- 25% after 112 days on the cholesterol-enriched diet. The measurements of surface area involvement were corroborated also by a significantly lower, chemically determined cholesterol content of the aorta in LAR rabbits. Plasma lipids and lipoproteins were determined at baseline, after 21 and 42 days of cholesterol feeding, and at the time the animals were killed. The plasma cholesterol concentrations of cholesterol-fed HAR and LAR rabbits showed a 13-fold increase after 21 days and a 21-fold increase after 84 days on the cholesterol diet.(ABSTRACT TRUNCATED AT 250 WORDS)
在本报告中，我们描述了两种兔品系的培育过程，即高动脉粥样硬化反应（HAR）品系和低动脉粥样硬化反应（LAR）品系，以及它们在饮食诱导的高胆固醇血症水平相似的情况下，主动脉发生动脉粥样硬化的倾向。随机选取62只雄性新西兰白兔，给予富含胆固醇的饮食（0.5%）84天，主动脉表面出现57±25%的嗜苏丹病变；12只兔子表现出低动脉粥样硬化反应（表面受累0%至30%），22只兔子表现出高动脉粥样硬化反应（表面受累70%至100%）。低动脉粥样硬化反应兔子的后代用于培育抗动脉粥样硬化兔品系（LAR品系），而高动脉粥样硬化反应兔子的后代用于培育HAR品系。第4代后开始进行控制育种，并在随后的6代中持续进行。因此，在LAR兔中，喂食胆固醇112天后，主动脉脂质可染色表面积仅为27±17%。另一方面，在HAR兔中，喂食富含胆固醇的饮食112天后，主动脉表面受累达85±25%。LAR兔主动脉中化学测定的胆固醇含量显著降低，也证实了表面积受累的测量结果。在基线、喂食胆固醇21天和42天后以及处死动物时测定血浆脂质和脂蛋白。喂食胆固醇的HAR兔和LAR兔的血浆胆固醇浓度在胆固醇饮食21天后增加了13倍，84天后增加了21倍。（摘要截短于250字）
