Lotze A, Stroud C Y, Soldin S J
Department of Neonatology, Children's National Medical Center, Washington, DC, 20010, USA.
Clin Chem. 1995 Aug;41(8 Pt 1):1182-8.
Serial tracheal aspirate samples were obtained for determination of lecithin/sphingomyelin (L/S) ratios from 47 term infants in respiratory failure. Phospholipids were extracted with Folch solution (chloroform:methanol, 2:1 by vol) and analyzed by HPLC with use of silica column and a mobile phase of acetonitrile:methanol:water (48:31:21, by vol). Surfactant/albumin (S/A) ratios were determined with the TDx Fetal Lung Maturity Assay (Abbott Labs). L/S ratios increased significantly over time in all patients (F = 19.42, P < 0.0001). The S/A ratio correlated with the L/S ratio (r = 0.554, P < 0.001). This study suggests that postnatal surfactant deficiency in term newborns with respiratory failure is a component of the newborn's lung injury.
从47名足月呼吸衰竭婴儿中获取系列气管吸出物样本，以测定卵磷脂/鞘磷脂（L/S）比值。用Folch溶液（氯仿：甲醇，体积比2:1）提取磷脂，并使用硅胶柱和乙腈：甲醇：水（体积比48:31:21）的流动相通过高效液相色谱法进行分析。用TDx胎儿肺成熟度测定法（雅培实验室）测定表面活性剂/白蛋白（S/A）比值。所有患者的L/S比值随时间显著增加（F = 19.42，P < 0.0001）。S/A比值与L/S比值相关（r = 0.554，P < 0.001）。本研究表明，足月呼吸衰竭新生儿出生后表面活性剂缺乏是新生儿肺损伤的一个组成部分。
