传统培养技术相较于采用光学免疫测定法快速检测A组链球菌的优越性。
Superiority of conventional culture technique over rapid detection of group A Streptococcus by optical immunoassay.
作者信息
Baker D M, Cooper R M, Rhodes C, Weymouth L A, Dalton H P
机构信息
Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298, USA.
出版信息
Diagn Microbiol Infect Dis. 1995 Feb;21(2):61-4. doi: 10.1016/0732-8893(95)00023-4.
An optical immunoassay (OIA) has been reported to be more sensitive than conventional culture for the detection of Group A Streptococcus, eliminating the need for culture. We attempted to confirm the sensitivity and specificity through a laboratory quantitation study and a clinical trial. OIA did not detect Group A Streptococcus below 10(5) colony forming units (CFU). Culture detected Streptococcus to 10(2) CFU from the inoculated swab. In the clinical study, throat swabs were obtained from 77 patients in an outpatient clinic. Compared with culture, the sensitivity of OIA was 78% and the specificity was 90%. These results demonstrate that OIA was less sensitive than culture in seeded experiments and missed 22% of positives in clinical practice. Our study, contrary to previous reports, suggests that OIA is not sensitive enough to be used as the sole assay for Group A Streptococcus pharyngitis.
据报道，一种光学免疫测定法（OIA）在检测A组链球菌方面比传统培养法更敏感，无需进行培养。我们试图通过实验室定量研究和临床试验来确认其敏感性和特异性。OIA检测不到低于10⁵菌落形成单位（CFU）的A组链球菌。培养法能从接种的拭子中检测到低至10²CFU的链球菌。在临床研究中，从一家门诊诊所的77名患者身上获取了咽喉拭子。与培养法相比，OIA的敏感性为78%，特异性为90%。这些结果表明，在接种实验中OIA不如培养法敏感，在临床实践中遗漏了22%的阳性病例。与之前的报道相反，我们的研究表明，OIA的敏感性不足以作为A组链球菌性咽炎的唯一检测方法。
Zotero 插件