Harbeck R J, Teague J, Crossen G R, Maul D M, Childers P L
National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
J Clin Microbiol. 1993 Apr;31(4):839-44. doi: 10.1128/jcm.31.4.839-844.1993.
A novel immunoassay system based on the changes in the reflection of light, termed an optical immunoassay (OIA), was utilized to directly detect group A streptococcal (GAS) carbohydrate antigen from clinical specimens. In two studies, a total of 1,275 throat swabs were tested for the presence of this antigen with the Strep A OIA rapid detection system and the results were compared with those of standard culture methods. In both studies, the Strep A OIA yielded more positive results than plating of the throat swab onto a selective agar, Trypticase soy agar containing sheep blood, or an enriched broth. In one study, the sensitivity and specificity of Strep A OIA compared with those of the broth-enriched culture were 97.4 and 95.6%, respectively. In a second study a sensitivity of 98.9% and a specificity of 98.6% were achieved. It was also shown that the carbohydrate antigen could be detected in the absence of viable GAS organisms. The Strep A OIA is an easily interpretable method and was shown to be more sensitive than routine culture methods for detecting GAS infections directly from throat swabs.
一种基于光反射变化的新型免疫分析系统，称为光学免疫分析（OIA），被用于直接检测临床标本中的A组链球菌（GAS）碳水化合物抗原。在两项研究中，使用A群链球菌OIA快速检测系统对总共1275份咽拭子进行了该抗原检测，并将结果与标准培养方法的结果进行了比较。在两项研究中，A群链球菌OIA产生的阳性结果均多于将咽拭子接种到选择性琼脂、含羊血的胰蛋白酶大豆琼脂或增菌肉汤上的结果。在一项研究中，与增菌肉汤培养相比，A群链球菌OIA的敏感性和特异性分别为97.4%和95.6%。在第二项研究中，敏感性达到98.9%，特异性达到98.6%。研究还表明，在没有活的GAS菌的情况下也能检测到碳水化合物抗原。A群链球菌OIA是一种易于解读的方法，并且已证明在直接从咽拭子检测GAS感染方面比常规培养方法更敏感。
