Navajas M, Gutierrez J, Bonato O, Bolland H R, Mapangou-Divassa S
Exp Appl Acarol. 1994 Jun;18(6):351-60. doi: 10.1007/BF00116316.
Intraspecific diversity in Cassava Green Mite Mononychellus progresivus Doreste was examined using individuals collected in Benin and the Congo and in Columbia and Brazil. Comparisons were based on mitochondrial and ribosomal DNA sequences and the results of several cross-breeding experiments. Sequence variation was examined in a total of 1139 base pairs (bp) constituting the ITS2 ribosomal DNA (805 bp) and a fragment of the Cytochrome Oxidase I (COI) gene (334 bp). Sequence divergence is low, ranging from 0% to 2.1% for COI and from 0% to 0.4% for ITS2. Inter-strain comparisons have shown that the two African populations appear to be identical. They were similar to the Colombian population while the Brazilian population was clearly different. The data support the hypothesis of a single introduction of the species in the two African populations. Crossing experiments have shown partial hybrid sterility, suggesting a genetic incompatibility consistent with differences detected by sequence data. The results show the usefulness of molecular markers as a tool for determining taxonomic status and dispersion paths in spider mites.
利用从贝宁、刚果、哥伦比亚和巴西采集的木薯绿螨（Mononychellus progresivus Doreste）个体，对其种内多样性进行了研究。比较基于线粒体和核糖体DNA序列以及若干杂交实验的结果。在构成ITS2核糖体DNA（805 bp）和细胞色素氧化酶I（COI）基因片段（334 bp）的总共1139个碱基对（bp）中检测序列变异。序列分歧较低，COI的范围为0%至2.1%，ITS2的范围为0%至0.4%。菌株间比较表明，两个非洲种群似乎是相同的。它们与哥伦比亚种群相似，而巴西种群明显不同。数据支持该物种在两个非洲种群中单次引入的假设。杂交实验表明存在部分杂种不育，这表明存在与序列数据检测到的差异一致的遗传不兼容性。结果表明分子标记作为确定叶螨分类地位和扩散路径的工具是有用的。
