Zhu Z, Tepel M, Neusser M, Zidek W
Medizinische Universitäts-Poliklinik, University of Münster, Germany.
Eur J Clin Invest. 1995 May;25(5):317-21. doi: 10.1111/j.1365-2362.1995.tb01708.x.
The modulatory effects of transforming growth factor beta 1 (TGF beta 1) on the angiotensin II (Ang II)-induced increase in cytosolic free calcium concentration ([Ca2+]i) were investigated in vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY). [Ca2+]i in VSMC was measured using the fluorescent dye fura-2. When TGF beta 1 was applied 30s prior to Ang II, the Ang II-induced [Ca2+]i increase was significantly enhanced in VSMC from SHR (P < 0.05 compared to control), whereas after the preincubation with TGF beta 1 for 30 min, the Ang II-induced [Ca2+]i increase was significantly reduced in VSMC from both strains. Using the manganese-quenching technique, it was confirmed that short-term exposure to TGF beta 1 enhanced the Ang II-induced trans-plasma-membrane calcium influx in SHR. The inhibition of protein kinase C by calphostin C abolished the stimulatory effect of TGF beta 1 on the Ang II-induced [Ca2+]i increase. It is concluded that TGF beta 1 modulates the Ang II-induced calcium handling in VSMC.
研究了转化生长因子β1（TGFβ1）对自发性高血压大鼠（SHR）和正常血压的Wistar-Kyoto大鼠（WKY）血管平滑肌细胞（VSMC）中血管紧张素II（Ang II）诱导的胞质游离钙浓度（[Ca2+]i）升高的调节作用。使用荧光染料fura-2测量VSMC中的[Ca2+]i。当在Ang II之前30秒应用TGFβ1时，SHR的VSMC中Ang II诱导的[Ca2+]i升高显著增强（与对照组相比，P < 0.05），而在用TGFβ1预孵育30分钟后，两种品系的VSMC中Ang II诱导的[Ca2+]i升高均显著降低。使用锰淬灭技术证实，短期暴露于TGFβ1可增强SHR中Ang II诱导的跨质膜钙内流。钙磷蛋白C对蛋白激酶C的抑制消除了TGFβ1对Ang II诱导的[Ca2+]i升高的刺激作用。得出结论，TGFβ1调节VSMC中Ang II诱导的钙处理。
