Gopaul N K, Anggård E E, Mallet A I, Betteridge D J, Wolff S P, Nourooz-Zadeh J
William Harvey Research Institute, St. Bartholomew's Hospital Medical College, London, UK.
FEBS Lett. 1995 Jul 17;368(2):225-9. doi: 10.1016/0014-5793(95)00649-t.
This study reports plasma levels of a specific nonenzymatic peroxidation product of arachidonic acid, esterified 8-epi-PGF2 alpha, from healthy- and NIDDM individuals as an index of oxidative stress in vivo. Plasma 8-epi-PGF2 alpha was isolated by solid-phase extraction on a C18 followed by an NH2 cartridge and analyzed by GC-MS/NICI as PFB-ester/TMS-ether derivative. We found that the average concentration of esterified 8-epi-PGF2 alpha among NIDDM subjects (0.93 +/- 0.07 nM, n = 39) was higher (P < 0.0001, Mann-Whitney test) than in healthy individuals (0.28 +/- 0.04 nM, n = 15). These data indicate that NIDDM is associated with increased plasma lipid peroxidation.
本研究报告了来自健康个体和非胰岛素依赖型糖尿病（NIDDM）个体的花生四烯酸特定非酶促过氧化产物——酯化8-表-前列腺素F2α的血浆水平，作为体内氧化应激的指标。血浆8-表-前列腺素F2α通过在C18柱上进行固相萃取，随后在NH2柱上进行萃取来分离，并通过气相色谱-质谱/负离子化学电离（GC-MS/NICI）分析其PFB-酯/TMS-醚衍生物。我们发现，NIDDM受试者中酯化8-表-前列腺素F2α的平均浓度（0.93±0.07 nM，n = 39）高于健康个体（0.28±0.04 nM，n = 15）（P < 0.0001，曼-惠特尼检验）。这些数据表明，NIDDM与血浆脂质过氧化增加有关。
