从嗜热栖热菌（Pyrococcus furiosus）中克隆并测序一个与大肠杆菌中编码磷酸烯醇丙酮酸合成酶的基因具有高度同源性的基因。

Cloning and sequencing of a gene from the archaeon Pyrococcus furiosus with high homology to a gene encoding phosphoenolpyruvate synthetase from Escherichia coli.

作者信息

Jones C E, Fleming T M, Piper P W, Littlechild J A, Cowan D A

机构信息

Department of Biochemistry and Molecular Biology, University College London, UK.

出版信息

Gene. 1995 Jul 4;160(1):101-3. doi: 10.1016/0378-1119(95)00128-s.

DOI:10.1016/0378-1119(95)00128-s

PMID:7628701

Abstract

A gene from the hyperthermophilic archaeon Pyrococcus furiosus, strain Vc1 (DSM 3638), contains an 817-amino-acid open reading frame which shows 42% identity to the phosphoenolpyruvate (PEP) synthetase of Escherichia coli. This putative P. furiosus PEP synthetase is slightly larger than the E. coli enzyme, the region between residues 58 and 89 being absent from the latter.

摘要

来自嗜热古菌激烈火球菌（Pyrococcus furiosus）Vc1菌株（DSM 3638）的一个基因含有一个817个氨基酸的开放阅读框，该开放阅读框与大肠杆菌的磷酸烯醇丙酮酸（PEP）合成酶具有42%的同一性。这种推测的激烈火球菌PEP合成酶比大肠杆菌的酶略大，后者缺少58至89位氨基酸之间的区域。