嗜热古菌激烈火球菌中编码一种极其耐热的[4Fe-4S]铁氧化还原蛋白的基因的克隆、表达及分子特性分析
Cloning, expression, and molecular characterization of the gene encoding an extremely thermostable [4Fe-4S] ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus.
作者信息
Heltzel A, Smith E T, Zhou Z H, Blamey J M, Adams M W
机构信息
Department of Biochemistry, University of Georgia, Athens 30602.
出版信息
J Bacteriol. 1994 Aug;176(15):4790-3. doi: 10.1128/jb.176.15.4790-4793.1994.
Abstract
The gene for ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus was cloned, sequenced, and expressed in Escherichia coli. The coding region confirmed the determined amino acid sequence. Putative archaeon-type transcriptional regulatory elements were identified. The fdxA gene appears to be an independent transcriptional unit. Recombinant ferredoxin was indistinguishable from the protein purified from P. furiosus in its thermal stability and in the potentiometric and spectroscopic properties of its [4Fe-4S] cluster.
摘要
对嗜热古菌激烈火球菌（Pyrococcus furiosus）的铁氧化还原蛋白基因进行了克隆、测序，并在大肠杆菌中进行了表达。编码区证实了所确定的氨基酸序列。鉴定出了假定的古菌型转录调控元件。fdxA基因似乎是一个独立的转录单元。重组铁氧化还原蛋白在热稳定性以及其[4Fe-4S]簇的电位和光谱特性方面与从激烈火球菌中纯化的蛋白质没有区别。
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