产黄青霉phoG的序列与结构，与构巢曲霉的一个酸性磷酸酶编码基因同源。

Sequence and structure of Penicillium chrysogenum phoG, homologous to an acid phosphatase-encoding gene of Aspergillus nidulans.

作者信息

Marx F, Haas H, Hofer S, Stöffler G, Redl B

机构信息

Institut für Mikrobiologie (Medizinische Fakultät), Universität Innsbruck, Austria.

出版信息

Gene. 1995 Jul 4;160(1):137-8. doi: 10.1016/0378-1119(95)00245-2.

DOI:10.1016/0378-1119(95)00245-2

PMID:7628710

Abstract

A Penicillium chrysogenum (Pc) gene (phoG), homologous to an Aspergillus nidulans (An) gene which confers phosphate-non-repressible acid phosphatase (APase) activity, has been cloned and sequenced. The 2.9-kb genomic sequence corresponds to two ORFs of 149 and 1630 bp encoding a protein of 593 amino acids (aa). As verified by cDNA sequencing, the coding region is interrupted by an 85-bp intron. The deduced aa sequence of phoG reveals 61% aa identity to the translated long ORF of the An APase-encoding gene. Northern blot analysis indicated a 2.3-kb transcript in approximately equivalent amount in mycelia grown under different phosphate concentrations.

摘要

已克隆并测序了产黄青霉（Pc）中一个与构巢曲霉（An）基因同源的基因（phoG），该构巢曲霉基因可赋予非磷酸盐阻遏酸性磷酸酶（APase）活性。2.9kb的基因组序列对应于两个开放阅读框，分别为149bp和1630bp，编码一个含593个氨基酸（aa）的蛋白质。经cDNA测序验证，编码区被一个85bp的内含子打断。phoG推导的氨基酸序列与An APase编码基因翻译后的长开放阅读框有61%的氨基酸同一性。Northern印迹分析表明，在不同磷酸盐浓度下生长的菌丝体中，有一个约2.3kb的转录本，其含量大致相当。