Martínez-Blanco H, Orejas M, Reglero A, Luengo J M, Peñalva M A
Centro de Investigaciones Biológicas del C.S.I.C., Madrid, Spain.
Gene. 1993 Aug 25;130(2):265-70. doi: 10.1016/0378-1119(93)90429-7.
Acetyl-coenzyme A synthetase (ACS; EC 6.2.1.1) from some plectomycete fungi is possibly involved in an accessory step of penicillin biosynthesis, in addition to its role in primary metabolism. We present the characterisation of the gene encoding this enzyme in Penicillium chrysogenum, which we designated acuA. Sequencing of genomic and cDNA clones showed that the coding region was interrupted by five introns, located at the same positions as those present in the Aspergillus nidulans homologue. This supports the possibility that the gene acquired its definitive mosaic organisation before the Penicillium/Aspergillus divergence. The mature transcript encodes a polypeptide with an M(r) of 74,287 which is 89.4% identical to its A. nidulans counterpart.
某些盘菌纲真菌中的乙酰辅酶A合成酶（ACS；EC 6.2.1.1）除了在初级代谢中发挥作用外，可能还参与青霉素生物合成的一个辅助步骤。我们对产黄青霉中编码该酶的基因进行了表征，将其命名为acuA。基因组和cDNA克隆的测序表明，编码区被五个内含子打断，这些内含子的位置与构巢曲霉同源物中的内含子位置相同。这支持了该基因在青霉属/曲霉属分化之前就获得了其最终镶嵌结构的可能性。成熟转录本编码一种分子量为74,287的多肽，与构巢曲霉的对应多肽有89.4%的同一性。
