West S E, Romero M J, Regassa L B, Zielinski N A, Welch R A
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706, USA.
Gene. 1995 Jul 4;160(1):81-6. doi: 10.1016/0378-1119(95)00236-y.
We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZ alpha-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.
我们构建了几种克隆载体，命名为pGZRS - 18/19和pGZRS - 38/39，它们基于胸膜肺炎放线杆菌（Apl）的一个4.3 kb的内源质粒。它们携带来自pUC18/19的lacZα - 互补片段和多克隆位点（MCS），以及在假定的Apl启动子控制下的bla基因或来自Tn903的KmR基因。这些载体能在Apl血清型1和7的代表性菌株、大肠杆菌、溶血巴斯德氏菌（Ph）和伴放线放线杆菌（放线杆菌属）中复制。我们还发现，Apl和Ph在lacZ或bla启动子控制下不表达基因，这表明它们的RNA聚合酶可能无法利用这些启动子。
