用于多杀性巴氏杆菌和大肠杆菌之间穿梭的载体构建。

Construction of a shuttle vector for use between Pasteurella multocida and Escherichia coli.

作者信息

Bills M M, Medd J M, Chappel R J, Adler B

机构信息

Department of Microbiology, Monash University, Clayton, Victoria, Australia.

出版信息

Plasmid. 1993 Nov;30(3):268-73. doi: 10.1006/plas.1993.1058.

DOI:10.1006/plas.1993.1058

PMID:8302934

Abstract

The isolation of a small plasmid from Pasteurella multocida has enabled the construction of a shuttle vector for use between P. multocida and Escherichia coli. The vector pBAC64 contains the origin of replication from P. multocida, an antibiotic resistance gene which functions in P. multocida, and the E. coli vector pUC18. The presence of the pUC18 multiple cloning site together with the lacZ' gene provides a screening method and allows cloning and manipulation in E. coli as well as cloning in P. multocida.

摘要

从多杀性巴氏杆菌中分离出一个小质粒，这使得构建一种可在多杀性巴氏杆菌和大肠杆菌之间使用的穿梭载体成为可能。载体pBAC64含有多杀性巴氏杆菌的复制起点、一个在多杀性巴氏杆菌中起作用的抗生素抗性基因以及大肠杆菌载体pUC18。pUC18多克隆位点与lacZ'基因的存在提供了一种筛选方法，并允许在大肠杆菌中进行克隆和操作，以及在多杀性巴氏杆菌中进行克隆。