Shapiro A B, Ling V
Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, Canada.
J Bioenerg Biomembr. 1995 Feb;27(1):7-13. doi: 10.1007/BF02110325.
Since P-glycoprotein was discovered almost 20 years ago, its causative role in multidrug resistance has been established, but central problems of its biochemistry have not been definitively resolved. Recently, major advances have been made in P-glycoprotein biochemistry with the use of purified and reconstituted P-glycoprotein, as well as membranes from nonmammalian cells containing heterologously expressed P-glycoprotein. In this review we describe recent findings using these systems which are elucidating the molecular mechanism of P-glycoprotein-mediated drug transport.
自从大约20年前发现P-糖蛋白以来，其在多药耐药性中的致病作用已得到确立，但其生物化学的核心问题尚未得到最终解决。最近，利用纯化和重组的P-糖蛋白以及含有异源表达P-糖蛋白的非哺乳动物细胞膜，P-糖蛋白生物化学取得了重大进展。在这篇综述中，我们描述了使用这些系统的最新发现，这些发现正在阐明P-糖蛋白介导的药物转运的分子机制。
