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A novel and simple methodology to generate subtracted cDNA libraries.一种用于生成消减cDNA文库的新颖且简单的方法。
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Solid phase subtractive cloning in differentially expressed genes identification.固相消减克隆在差异表达基因鉴定中的应用。
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Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy.采用改良的cDNA末端快速扩增策略对印度主要鲤鱼生长激素编码cDNA进行分子克隆
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A novel ligation mediated-PCR based strategy for construction of subtraction libraries from limiting amounts of mRNA.一种基于连接介导的聚合酶链反应的新型策略，用于从有限量的信使核糖核酸构建消减文库。
Nucleic Acids Res. 1996 Feb 15;24(4):795-6. doi: 10.1093/nar/24.4.795.

本文引用的文献

1
Cell-type-specific cDNA probes and the murine I region: the localization and orientation of Ad alpha.细胞类型特异性cDNA探针与小鼠I区：Adα的定位与方向
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2194-8. doi: 10.1073/pnas.81.7.2194.
2
Changes in brain gene expression shared by scrapie and Alzheimer disease.瘙痒病和阿尔茨海默病共有的大脑基因表达变化。
Proc Natl Acad Sci U S A. 1989 Sep;86(18):7260-4. doi: 10.1073/pnas.86.18.7260.
3
Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.通过cDNA文库的消减杂交分离瘙痒病调节RNA的cDNA
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5738-42. doi: 10.1073/pnas.85.15.5738.
4
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.采用酸性硫氰酸胍-苯酚-氯仿萃取法一步分离RNA的方法。
Anal Biochem. 1987 Apr;162(1):156-9. doi: 10.1006/abio.1987.9999.
5
Oncogenic conversion by regulatory changes in transcription factors.
Cell. 1991 Jan 25;64(2):303-12. doi: 10.1016/0092-8674(91)90640-k.
6
Construction of a cDNA library from microdissected guinea pig organ of Corti.从显微切割的豚鼠柯蒂氏器构建cDNA文库。
Hear Res. 1992 Sep;62(1):124-6. doi: 10.1016/0378-5955(92)90208-5.

A novel and simple methodology to generate subtracted cDNA libraries.

作者信息

Rivolta M N, Wilcox E R

机构信息

Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, MD 20850, USA.

出版信息

Nucleic Acids Res. 1995 Jul 11;23(13):2565-6. doi: 10.1093/nar/23.13.2565.

DOI:10.1093/nar/23.13.2565
PMID:7630738
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307068/
Abstract
摘要