Duguid J R, Rohwer R G, Seed B
GRECC, Edith Nourse Rogers Memorial Veterans Administration Hospital, Bedford, MA 01730.
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5738-42. doi: 10.1073/pnas.85.15.5738.
We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in pi H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brain. DNA sequence analysis showed that they represent glial fibrillary acidic protein, metallothionein II, and the B chain of alpha-crystallin; the latter two may represent a response to stress.
我们基于在pi H3M（一种含有噬菌体M13复制起点的载体）中构建的单链cDNA文库的杂交，开发了一种消减克隆程序。我们已使用该策略分离出三种在瘙痒病感染的大脑中丰度增加的转录本。DNA序列分析表明，它们分别代表胶质纤维酸性蛋白、金属硫蛋白II和α-晶状体蛋白的B链；后两者可能代表对应激的一种反应。
