Bertuglia S, Malandrino S, Colantuoni A
CNR Institute of Clinical Physiology, Pisa, Italy.
Pharmacol Res. 1995 Mar-Apr;31(3-4):183-7. doi: 10.1016/1043-6618(95)80016-6.
The effects of Vaccinium myrtillus anthocyanosides (VMA) on ischaemia reperfusion injury were investigated in the hamster cheek pouch microcirculation. Ischaemia was induced by clamping the cheek pouch for 30 min followed by 30 min of reperfusion. The microvasculature was visualized by a fluorescence technique. VMA [10 mg (100 g body weight)-1] were orally administered for 2 and 4 weeks. The number of adhering leukocytes to venular vessel walls, the perfused capillary length, the increase in permeability, the arteriolar diameter changes were determined. Ischaemia and reperfusion were associated with increased number of leukocytes sticking to venules, decreased number of perfused capillaries, and increased permeability. VMA decreased the number of leukocytes sticking to the venular wall and preserved the capillary perfusion; the increase in permeability was significantly reduced after reperfusion. VMA saved the arteriolar tone and induced the appearance of rhythmic diameter changes of arterioles. These results demonstrate the ability of Vaccinium myrtillus anthocyanosides to reduce microvascular impairments due to ischaemia reperfusion injury, with preservation of endothelium, attenuation of leukocyte adhesion and improvement of capillary perfusion.
在仓鼠颊囊微循环中研究了越橘花青素（VMA）对缺血再灌注损伤的影响。通过夹闭颊囊30分钟诱导缺血，随后再灌注30分钟。采用荧光技术观察微血管系统。VMA[10毫克/（100克体重）-1]口服给药2周和4周。测定黏附于微静脉血管壁的白细胞数量、灌注的毛细血管长度、通透性增加情况以及小动脉直径变化。缺血和再灌注与黏附于微静脉的白细胞数量增加、灌注的毛细血管数量减少以及通透性增加有关。VMA减少了黏附于微静脉壁的白细胞数量并维持了毛细血管灌注；再灌注后通透性增加显著降低。VMA维持了小动脉张力并诱导小动脉出现有节律的直径变化。这些结果表明，越橘花青素能够减轻缺血再灌注损伤导致的微血管损伤，具有保护内皮、减轻白细胞黏附以及改善毛细血管灌注的作用。
