Steenstrup B R, Alm P, Hannibal J, Jørgensen J C, Palle C, Junge J, Christensen H B, Ottesen B, Fahrenkrug J
Department of Gynaecology and Obstetrics, Hvidovre Hospital, Sweden.
Am J Physiol. 1995 Jul;269(1 Pt 1):E108-17. doi: 10.1152/ajpendo.1995.269.1.E108.
The distribution, localization, and smooth muscle effects of pituitary adenylate cyclase-activating polypeptide (PACAP) were studied in the human female genital tract. The concentrations of PACAP-38 and PACAP-27 were measured by radioimmunoassays, and both peptides were found throughout the genital tract. The highest concentrations of PACAP-38 were detected in the ovary, the upper part of vagina, and the perineum. The concentrations of PACAP-27 were generally low, in some regions below the detection limit and in other regions 1 to 5% of the PACAP-38 concentrations. Immunocytochemistry revealed that PACAP was located in delicate varicose nerve fibers that were most abundant in the internal cervical os, where they mainly seemed to innervate blood vessels and smooth muscle cells. PACAP-38 and PACAP-27 (10(-10)-10(-6) M) caused a concentration-dependent relaxation of the spontaneous activity of the nonvascular smooth muscle strips from fallopian tube and myometrium in vitro. Likewise, both peptides (10(-10)-10(-6) M) caused relaxation of nonrepinephrine (10(-6) M)-precontracted intramyometrial arteries. No effect of the PACAP sequences, PACAP-(6-27), PACAP-(16-38), and PACAP-(18-27), on fallopian tube was observed. The findings suggest a smooth muscle regulatory role of PACAP in the human female reproductive tract.
研究了垂体腺苷酸环化酶激活多肽（PACAP）在女性生殖道中的分布、定位及对平滑肌的作用。采用放射免疫分析法测定了PACAP - 38和PACAP - 27的浓度，发现这两种肽存在于整个生殖道中。在卵巢、阴道上段和会阴处检测到最高浓度的PACAP - 38。PACAP - 27的浓度普遍较低，在某些区域低于检测限，在其他区域为PACAP - 38浓度的1%至5%。免疫细胞化学显示，PACAP位于纤细的曲张神经纤维中，这些神经纤维在子宫颈内口最为丰富，主要似乎支配血管和平滑肌细胞。PACAP - 38和PACAP - 27（10⁻¹⁰ - 10⁻⁶ M）在体外引起输卵管和子宫肌层非血管平滑肌条自发活动的浓度依赖性松弛。同样，这两种肽（10⁻¹⁰ - 10⁻⁶ M）引起去甲肾上腺素（10⁻⁶ M）预收缩的子宫肌层内动脉的松弛。未观察到PACAP序列PACAP - (6 - 27)、PACAP - (16 - 38)和PACAP - (18 - 27)对输卵管有影响。这些发现提示PACAP在女性生殖系统中对平滑肌具有调节作用。
