Contreras M C, Gallo S, Salinas P, Sapunar J, Sandoval L, Solís F
Departamento de Parasitología, Facultad de Medicina, Universidad de Chile, Santiago.
Bol Chil Parasitol. 1994 Jan-Jun;49(1-2):24-30.
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of IgG antibodies to purified sheep hydatic cyst fluid antigen in 56 sera of confirmed cases of hydatidosis. The cut-off value was determined using serum samples from 80 healthy persons, employing two serum dilutions (1:100 and 1:500) with two and three standard deviations (SD). This assay was compared with the indirect hemagglutination test (IHAT). The sensitivity of ELISA-IgG was 94.3% for hepatic cysts and 92.9% for pulmonary cysts, whereas the values for IHAT were 77.1 and 64.3% respectively. According to Mac Nemar test, both method presented statistical significance (p < 0.05). In order to find out the specificity, additional 70 serum samples from individuals with other parasitoses, such as cysticercosis (30), trichinosis (26) and fascioliasis (14) were also tested. IHAT presented a specificity of 92.7% and for ELISA-IgG the specificity using a cut-off of mean + 3 SD was 99.3 and 100.0% with sera dilution of 1:100 and 1:500 respectively when a cut-off of mean + 2 SD was considered, we found a specificity of 91.3 and 97.3% for 1:100 and 1:500 dilutions. The use of ELISA-IgG and purified antigen in the diagnosis of human hydatidosis is discussed.
开发了一种酶联免疫吸附测定（ELISA）法，用于检测56例确诊包虫病患者血清中针对纯化羊囊尾蚴液抗原的IgG抗体。使用80名健康人的血清样本，采用两种血清稀释度（1:100和1:500）以及两个和三个标准差（SD）来确定临界值。将该测定法与间接血凝试验（IHAT）进行比较。ELISA-IgG对肝囊肿的敏感性为94.3%，对肺囊肿的敏感性为92.9%，而IHAT的值分别为77.1%和64.3%。根据麦克内马尔检验，两种方法均具有统计学意义（p < 0.05）。为了确定特异性，还检测了另外70份来自患有其他寄生虫病个体的血清样本，如囊尾蚴病（30例）、旋毛虫病（26例）和肝片吸虫病（14例）。IHAT的特异性为92.7%，对于ELISA-IgG，当临界值为均值 + 3 SD时，血清稀释度为1:100和1:500时的特异性分别为99.3%和100.0%；当考虑临界值为均值 + 2 SD时，1:100和1:500稀释度的特异性分别为91.3%和97.3%。讨论了ELISA-IgG和纯化抗原在人类包虫病诊断中的应用。
