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意大利三种本土寄生虫感染(弓蛔虫病、包虫病和片形吸虫病)中的放射变应原吸附试验和酶联免疫吸附测定

RAST and ELISA in three autochthonous parasitic infections in Italy: toxocariasis, hydatidosis and fascioliasis.

作者信息

Tinelli M, Falagiani P, Riva G, Genchi C

出版信息

Boll Ist Sieroter Milan. 1987;66(1):38-45.

PMID:3620121
Abstract

Toxocariasis, Hydatidosis and Fascioliasis are common autochthonous parasitic infections in Italy. RAST and ELISA have been evaluated for serological diagnosis. Eight sera from Toxocariasis, fifty from Hydatidosis and two from Fascioliasis have been examined. Excretory/secretory antigen has been employed for the serology of Toxocariasis, hydatic fluid for Hydatidosis and embryonated-eggs antigen for Fascioliasis. The RAST resulted positive in 4/5 cases of the visceral form of Toxocariasis and 3/3 in the ocular form, in 2/2 cases of Fascioliasis and 27/50 of Hydatidosis. The ELISA resulted positive respectively in 5/5, 3/3, 2/2, and 19/50 cases. The sensitivity and specificity of the tests employed have been proved.

摘要

弓蛔虫病、包虫病和肝片吸虫病是意大利常见的本地寄生虫感染。已对放射变应原吸附试验(RAST)和酶联免疫吸附测定(ELISA)进行了血清学诊断评估。检查了8份弓蛔虫病血清、50份包虫病血清和2份肝片吸虫病血清。排泄/分泌抗原已用于弓蛔虫病血清学检测,囊液用于包虫病血清学检测,受精虫卵抗原则用于肝片吸虫病血清学检测。RAST在5例内脏型弓蛔虫病中有4例呈阳性,在3例眼型弓蛔虫病中3例呈阳性,在2例肝片吸虫病中2例呈阳性,在50例包虫病中27例呈阳性。ELISA分别在5/5、3/3、2/2和19/50例中呈阳性。所采用检测方法的敏感性和特异性已得到验证。

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