Yu Z W, Tsvetkova N M, Tsonev L I, Quinn P J
Division of Life Sciences, King's College London, UK.
Biochim Biophys Acta. 1995 Jul 26;1237(2):135-42. doi: 10.1016/0005-2736(95)00097-m.
The phase behaviour of 1,2-distearoylphosphatidylethanolamine in glycerol has been examined using differential scanning calorimetry and real-time synchrotron X-ray diffraction methods. Dry phospholipid and phospholipid dispersed in glycerol over the concentration range 2.4%-90% (w/w) was equilibrated for 30 min at 20 degrees C and thermal and structural parameters on the temperature range 60 degrees C to 110 degrees C recorded during an initial heating and subsequent reheating. The characteristic feature of the initial heating scan was a direct lamellar crystalline to inverted hexagonal phase transition. In the subsequent cooling scan a lamellar gel structure was formed from the non-lamellar phase which transformed, on reheating, to a lamellar crystalline phase in which the acyl chain packing was titled with respect to the bilayer plane. The mechanism of the formation of the two crystalline phases was examined in the context of a relaxation model, where the liquid-crystal phase below the transition temperature from lamellar crystalline phase is metastable. A binary phase diagram over the temperature range 60 degrees C to 110 degrees C has been constructed.
利用差示扫描量热法和实时同步加速器X射线衍射法研究了1,2-二硬脂酰磷脂酰乙醇胺在甘油中的相行为。将干燥的磷脂以及分散于甘油中浓度范围为2.4%-90%（w/w）的磷脂在20℃下平衡30分钟，并在初始加热及随后的再加热过程中记录60℃至110℃温度范围内的热参数和结构参数。初始加热扫描的特征是从直接的层状晶体相向反相六角相转变。在随后的冷却扫描中，由非层状相形成了层状凝胶结构，该结构在再加热时转变为层状晶体相，其中酰基链堆积相对于双层平面发生了倾斜。在弛豫模型的背景下研究了两种晶体相的形成机制，其中低于层状晶体相转变温度的液晶相是亚稳态的。构建了60℃至110℃温度范围内的二元相图。
