一种鱼类醛缩酶B基因的克隆与特性分析

Cloning and characterisation of a fish aldolase B gene.

作者信息

Llewellyn L, Ramsurn V P, Sweeney G E, Wigham T, Santos C R, Power D M

机构信息

School of Molecular and Medical Biosciences, University of Wales, Cardiff, UK.

出版信息

Biochim Biophys Acta. 1995 Jul 25;1263(1):75-8. doi: 10.1016/0167-4781(95)00096-y.

DOI:10.1016/0167-4781(95)00096-y

PMID:7632737

Abstract

A full length cDNA clone representing an aldolase mRNA was isolated from a sea bream (Sparus aurata) liver cDNA library. Sequencing of this clone revealed it to encode a 364 amino acid protein with 74% amino acid identity to human aldolase B and slightly lower similarity to human aldolase A and C. In view of the sequence data and of Northern blot analysis showing strong expression of a 1.6 kb transcript in liver it was concluded that the cloned gene represents aldolase B. This clone represents the first aldolase gene to be sequenced from any fish species thus providing new data on the evolution of the vertebrate aldolase gene family.

摘要

从黑鲷肝脏cDNA文库中分离出一个代表醛缩酶mRNA的全长cDNA克隆。对该克隆进行测序后发现，它编码一种364个氨基酸的蛋白质，与人类醛缩酶B的氨基酸同一性为74%，与人类醛缩酶A和C的相似性略低。鉴于序列数据以及Northern印迹分析显示在肝脏中有一个1.6 kb转录本的强烈表达，得出结论该克隆基因代表醛缩酶B。这个克隆代表了第一个从任何鱼类物种中测序的醛缩酶基因，从而为脊椎动物醛缩酶基因家族的进化提供了新的数据。