Paolella G, Santamaria R, Izzo P, Costanzo P, Salvatore F
Nucleic Acids Res. 1984 Oct 11;12(19):7401-10. doi: 10.1093/nar/12.19.7401.
Two recombinant clones, pA2 and pA3, containing cDNA sequences for human aldolase B have been isolated from a full length human liver cDNA library. The larger one, pA3, has been subcloned in M13 phage and completely sequenced with the chain terminator method. The sequence covers 1,600 nucleotides including the whole coding region (1,050 nucleotides), 67 nucleotides from the 5' non-coding region and the whole 3' non-coding region, 440 nucleotides long, down to the poly-A tail. Comparison with rabbit aldolase A and with a partial sequence of rat aldolase B, shows a homology of about 76% for aldolase A and of about 94% for aldolase B, which indicates that the sequenced cDNA codes for the liver isoenzyme. This is the first complete sequence reported for human aldolase B. The pA3 clone strongly hybridizes to 18S mRNA from human adult liver as expected from the size of the isolated cDNA.
从一个全长人肝cDNA文库中分离出了两个含有人类醛缩酶B cDNA序列的重组克隆，即pA2和pA3。其中较大的克隆pA3已亚克隆到M13噬菌体中，并采用链终止法进行了全序列测定。该序列涵盖1600个核苷酸，包括整个编码区（1050个核苷酸）、5'非编码区的67个核苷酸以及整个3'非编码区（440个核苷酸长），直至聚腺苷酸尾。与兔醛缩酶A以及大鼠醛缩酶B的部分序列进行比较，结果显示醛缩酶A的同源性约为76%，醛缩酶B的同源性约为94%，这表明所测序的cDNA编码的是肝脏同工酶。这是首次报道的人类醛缩酶B的完整序列。正如从分离出的cDNA大小所预期的那样，pA3克隆与来自成人肝脏的18S mRNA强烈杂交。
