去纤维蛋白法在单核细胞中离子镁测定中至关重要。
Defibrination essential in the assay of ionized magnesium in mononuclear cells.
作者信息
Willems H L, Kruse J, Geven W B, Vis P, Monnens L A
机构信息
Afdeling Klinische Chemie van het Academisch Ziekenhuis Nijmegen, St. Radboud, Nijmegen, The Netherlands.
出版信息
Eur J Clin Chem Clin Biochem. 1995 Feb;33(2):103-4.
Intracellular free (ionized) magnesium concentration was measured in mononuclear cells isolated from healthy volunteers by use of dual wavelength fluorescence (indicator: mag-fura-2). We found a free Mg2+ concentration of 1.28 +/- 0.08 mmol/l in mononuclear cells isolated from heparinized blood. When we defibrinated blood samples prior to the isolation step we measured 0.78 +/- 0.05 mmol/l of free Mg2+ in these cells. We conclude that this difference is caused by the platelets present in the heparinized specimens.
采用双波长荧光法（指示剂：镁荧光素-2）测量从健康志愿者分离出的单核细胞中的细胞内游离（离子化）镁浓度。我们发现，从肝素化血液中分离出的单核细胞中游离Mg2+浓度为1.28±0.08 mmol/L。当我们在分离步骤之前对血样进行去纤维蛋白处理时，测得这些细胞中游离Mg2+浓度为0.78±0.05 mmol/L。我们得出结论，这种差异是由肝素化标本中存在的血小板引起的。
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