Yoshimura M, Oshima T, Matsuura H, Watanabe M, Higashi Y, Ono N, Hiraga H, Kanbe M, Kajiyama G
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
J Lab Clin Med. 1995 Jun;125(6):743-7.
We measured basal cytosolic concentration of free Mg2+ ([Mg2+]i) in platelets from 30 healthy volunteers by using mag-fura-2, a new fluorescent Mg2+ indicator. The mean [Mg2+]i was 381 +/- 22 mumol/L, with values ranging from 226 to 771 mumol/L. The day-to-day intrasubject coefficient of variation was relatively small (3.6%). [Mg2+]i was significantly higher in men than in women (430 +/- 38 vs 332 +/- 17 mumol/L, p < 0.03), and was not correlated with age. There was no significant relation between platelet [Mg2+]i and serum total Mg concentration. The results indicate that the gender but not age may decide intracellular Mg2+ status. In addition, the mechanisms that regulate [Mg2+]i may be independent of those that influence the extracellular concentration of Mg.
我们使用新型荧光镁离子指示剂mag-fura-2测量了30名健康志愿者血小板中游离镁离子（[Mg2+]i）的基础胞浆浓度。平均[Mg2+]i为381±22μmol/L，范围在226至771μmol/L之间。受试者自身每日的变异系数相对较小（3.6%）。男性的[Mg2+]i显著高于女性（430±38对332±17μmol/L，p<0.03），且与年龄无关。血小板[Mg2+]i与血清总镁浓度之间无显著关系。结果表明，决定细胞内镁离子状态的可能是性别而非年龄。此外，调节[Mg2+]i的机制可能独立于影响细胞外镁浓度的机制。
