Aalen R B
Division of General Genetics, University of Oslo, Norway.
Plant Mol Biol. 1995 Jun;28(3):583-8. doi: 10.1007/BF00020404.
Two transcripts (Ole-1 and Ole-2) encoding two oleosin isoforms homologous to the 18 and 16 kDa oleosins of maize, respectively, have been isolated from developing barley embryos and aleurone layers where lipid bodies are highly abundant organelles. For each of the isoforms the aleurone and embryo transcripts are identical, indicating that the same genes are expressed in both tissues. The temporal accumulation of the two transcripts during seed development is similar. At a low frequency, lipid bodies are found also in starchy endosperm cells of barley. Accordingly, a low transcript level is observed for both oleosins during starchy endosperm development.
从发育中的大麦胚和糊粉层中分离出了两种转录本(Ole-1和Ole-2),它们分别编码与玉米18 kDa和16 kDa油质蛋白同源的两种油质蛋白亚型,在这些组织中脂体是高度丰富的细胞器。对于每种亚型,糊粉层和胚的转录本是相同的,这表明相同的基因在这两种组织中都有表达。两种转录本在种子发育过程中的积累时间相似。在大麦的淀粉胚乳细胞中也能以低频率发现脂体。因此,在淀粉胚乳发育过程中,两种油质蛋白的转录本水平都很低。