Rootwelt T, Almaas R, Oyasaeter S, Moen A, Saugstad O D
Department of Pediatric Research, University of Oslo, Rikshospitalet, Norway.
Acta Paediatr. 1995 May;84(5):507-11. doi: 10.1111/j.1651-2227.1995.tb13683.x.
Xanthine oxidase may contribute to oxygen free radical formation during reoxygenation after hypoxia, but in humans the enzyme is present in substantial amounts only in the liver and intestine. We developed a sensitive assay for xanthine oxidase using 14C-xanthine as substrate and investigated whether xanthine oxidase was released into the systemic circulation when 19 newborn pigs were resuscitated after severe hypoxemia. In five piglets plasma xanthine oxidase concentrations increased from undetectable levels to a median value of 8 (range 4-18) microU/ml after 30 min of reoxygenation. In these pigs serum aspartate aminotransferase increased from 45 to 148 U/l, while alanine aminotransferase was unchanged (28-31 U/l). The release of xanthine oxidase did not seem to correlate with the severity of the histological brain damage after 4 days. We conclude that only low levels of xanthine oxidase are released to the systemic circulation after severe hypoxemia in newborn pigs.
黄嘌呤氧化酶可能在缺氧后的复氧过程中促使氧自由基形成，但在人类中，该酶仅大量存在于肝脏和肠道。我们开发了一种以14C - 黄嘌呤为底物的黄嘌呤氧化酶灵敏检测方法，并研究了19只新生猪在严重低氧血症后复苏时，黄嘌呤氧化酶是否释放到体循环中。在5只仔猪中，复氧30分钟后，血浆黄嘌呤氧化酶浓度从不可检测水平升至中位数8（范围4 - 18）微单位/毫升。在这些猪中，血清天冬氨酸转氨酶从45升至148 U/L，而丙氨酸转氨酶未变（28 - 31 U/L）。4天后，黄嘌呤氧化酶的释放似乎与组织学脑损伤的严重程度无关。我们得出结论，新生猪严重低氧血症后仅有少量黄嘌呤氧化酶释放到体循环中。
