Lowenberger C, Bulet P, Charlet M, Hetru C, Hodgeman B, Christensen B M, Hoffmann J A
University of Wisconsin--Madison 53706, USA.
Insect Biochem Mol Biol. 1995 Jul;25(7):867-73. doi: 10.1016/0965-1748(95)00043-u.
The injection of Escherichia coli and Micrococcus luteus into the hemocoel of Aedes aegypti induces a potent antibacterial activity in the hemolymph. We have purified and fully characterized three 40-residue antibacterial peptides from the hemolymph of bacteria-challenged mosquitoes that are absent in naive mosquitoes. The peptides are potently active against Gram-positive bacteria and against one of the Gram-negative bacteria that were tested. The amino acid sequences clearly show that the three peptides are novel isoforms of the insect defensin family of antibacterial peptides. They differ from each other by one or two amino acid residues. We present here the complete amino acid sequences of the three isoforms and the activity spectrum of the predominant Aedes defensin.
将大肠杆菌和藤黄微球菌注入埃及伊蚊的血腔会在其血淋巴中诱导出强大的抗菌活性。我们已从受细菌攻击的蚊子血淋巴中纯化并全面鉴定了三种由40个氨基酸残基组成的抗菌肽，未受感染的蚊子血淋巴中不存在这些肽。这些肽对革兰氏阳性菌以及所测试的一种革兰氏阴性菌具有强大的活性。氨基酸序列清楚地表明，这三种肽是昆虫防御素家族抗菌肽的新型异构体。它们彼此之间相差一或两个氨基酸残基。我们在此展示这三种异构体的完整氨基酸序列以及主要的埃及伊蚊防御素的活性谱。
