Shibusawa Y, Suzuki K, Kinoshita H, Matsumoto U
Department of Analytical Chemistry, Tokyo College of Pharmacy, Japan.
J Chromatogr B Biomed Appl. 1995 Apr 21;666(2):233-9. doi: 10.1016/0378-4347(94)00580-x.
Selective separation of human peripheral platelets, granulocytes and lymphocytes was investigated by column liquid chromatography using methoxyethoxymethyl (MEM) bonded-phase columns (25 x 0.9 cm I.D.). Isotonic solutions containing mono- and disaccharides, methyl-alpha-D-pyranosides and a physiological saline at pH 7.4 were used as the mobile phase. Granulocytes and lymphocytes were separated on the MEM-Cellulofine GH-25 column by elution with 0.3 M D-mannose solution. The isolation of platelets and lymphocytes from human leukocyte-rich plasma was performed with a MEM-Sephadex G25 column and elution with 0.27 M sucrose solution. On the same column platelets could also be collected selectively by elution with 0.31 M methyl-alpha-D-mannoside at the high recovery of 100%. The isolated cells were viable for more than 90%.
使用甲氧基乙氧基甲基（MEM）键合相柱（内径25×0.9 cm）通过柱液相色谱法研究了人外周血血小板、粒细胞和淋巴细胞的选择性分离。含有单糖和二糖、α-D-吡喃甲基糖苷的等渗溶液以及pH 7.4的生理盐水用作流动相。粒细胞和淋巴细胞在MEM-Cellulofine GH-25柱上用0.3 M D-甘露糖溶液洗脱进行分离。用MEM-葡聚糖凝胶G25柱从富含人白细胞的血浆中分离血小板和淋巴细胞，并用0.27 M蔗糖溶液洗脱。在同一柱上，血小板也可以通过用0.31 Mα-D-甲基甘露糖苷洗脱以100%的高回收率选择性收集。分离出的细胞存活率超过90%。
