Matsumoto U, Shibusawa Y
J Chromatogr. 1980 Jan 18;187(2):351-62. doi: 10.1016/s0021-9673(00)80467-7.
Two different types of column packings with bonded polyethylene glycol (PEG) as the stationary phase, PEG 20M-bonded Porasil AX and bisoxirane-coupled PEG 20M-Sepharose 6B, were prepared for chromatographing human and rabbit peripheral blood cells. The best separation, especially of granulocytes and lymphocytes (separation factor 2.65), was obtained with the latter packing and a phosphate-buffered solution (pH 7.5) of 8% (w/w) dextran as the mobile phase. The selectivity of the column for blood cells depended on the concentration and molecular weight of dextran and on the ionic composition of the electrolytes in the mobile phase. The recoveries of human granulocytes, lymphocytes and erythrocytes by this chromatographic system were about 67, 82 and 50%, respectively.
制备了两种以键合聚乙二醇（PEG）为固定相的不同类型的柱填料，即PEG 20M键合的Porasil AX和双环氧乙烷偶联的PEG 20M-琼脂糖6B，用于分离人和兔的外周血细胞。使用后一种填料和8%（w/w）葡聚糖的磷酸盐缓冲溶液（pH 7.5）作为流动相时，获得了最佳分离效果，尤其是粒细胞和淋巴细胞的分离（分离因子为2.65）。柱对血细胞的选择性取决于葡聚糖的浓度和分子量以及流动相中电解质的离子组成。通过该色谱系统对人粒细胞、淋巴细胞和红细胞的回收率分别约为67%、82%和50%。
