Sträter J, Günthert A R, Brüderlein S, Möller P
Institute of Pathology, University of Heidelberg, Germany.
Histochem Cell Biol. 1995 Feb;103(2):157-60. doi: 10.1007/BF01454013.
Apoptosis is a morphologically distinct form of programmed cell death that plays an important role in the growth regulation of a variety of tissues and also in the elimination of self-reacting immunocompetent cells. Several techniques for the qualitative and quantitative detection of this process have been established; recently, an in situ nick end-labelling technique based on the detection of DNA fragmentation, which is a molecular characteristic of apoptotic cell death, was described. Applying this method to paraffin sections of human tissues, sensitivity was observed to be inconsistently low with regard to the expected number of apoptotic cells. In the present study we show that irradiation of the tissue sections in 10 mM citrate buffer, pH 6.0, by microwaves at 750 W considerably enhances the sensitivity of this nick end-labelling technique.
细胞凋亡是一种形态学上独特的程序性细胞死亡形式，在多种组织的生长调节以及清除自身反应性免疫活性细胞中发挥着重要作用。已经建立了几种定性和定量检测这一过程的技术；最近，描述了一种基于检测DNA片段化的原位缺口末端标记技术，DNA片段化是凋亡细胞死亡的分子特征。将该方法应用于人体组织的石蜡切片时，观察到对于预期凋亡细胞数量而言，敏感性一直较低。在本研究中，我们表明在pH 6.0的10 mM柠檬酸盐缓冲液中，用750 W微波照射组织切片可显著提高这种缺口末端标记技术的敏感性。
