Ito T, Sakano S, Sato K, Sugiura H, Iwata H, Murata Y, Seo H
Department of Orthopaedic Surgery, School of Medicine, Nagoya, Japan.
Clin Orthop Relat Res. 1995 Jul(316):267-75.
For autogenous and allogeneic bone grafts, heat treatment has been thought to kill malignant cells and viruses such as human immunodeficiency virus. It is unclear whether heat treatment could preserve bone-inductive activity. Cortical bones from 6-week-old rat femurs were heated in a water bath at a temperature of 50 degrees-100 degrees C for periods of 15 minutes to 10 hours. After treatment, they were defatted and decalcified. Each sample was transplanted into the hamstring muscle of 3-week-old rats. Eleven days after transplantation, the samples were removed and messenger ribonucleic acids (mRNAs) were determined for alkaline phosphatase and collagens in the transplant. Twenty-one days after transplantation, actual bone formation was studied by histologic analysis and measurement of calcium content. Heat treatment at 60 degrees C for 10 hours and at 70 degrees C for 1 hour preserved bone-inductive activity, as indicated by the induction of mRNAs for alkaline phosphatase and Type I and Type II collagens. Significant decreased in Type II collagen mRNA and calcium content were observed at 70 degrees C when the transplants were heated for 10 hours, suggesting the importance of evaluating the duration of heat treatment.
对于自体骨移植和异体骨移植，热处理被认为可以杀死恶性细胞和病毒，如人类免疫缺陷病毒。目前尚不清楚热处理是否能保留骨诱导活性。将6周龄大鼠股骨的皮质骨在50℃至100℃的水浴中加热15分钟至10小时。处理后，将其脱脂和脱钙。每个样本被移植到3周龄大鼠的腿筋肌肉中。移植11天后，取出样本并测定移植组织中碱性磷酸酶和胶原蛋白的信使核糖核酸（mRNA）。移植21天后，通过组织学分析和钙含量测量研究实际的骨形成情况。60℃加热10小时和70℃加热1小时能保留骨诱导活性，这可通过碱性磷酸酶以及I型和II型胶原蛋白的mRNA诱导来表明。当移植组织在70℃加热10小时时，观察到II型胶原蛋白mRNA和钙含量显著降低，这表明评估热处理持续时间的重要性。
