Hazan R, Oron U
Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel.
J Orthop Res. 1993 Sep;11(5):655-63. doi: 10.1002/jor.1100110507.
The kinetics of growth of bone into control (nontreated) and heat-treated screws made of stainless steel (type 316L) and Ti-6Al-4V, implanted in the medullary canal of the femur in rats, were studied by mechanical, histological, and biochemical methods. A progressive and significant increase in the ingrowth of bone, as reflected by interfacial shear strengths of the screws, was measured with time after implantation. At all time intervals for as long as 35 days after implantation, the shear strength of the heat-treated Ti-6Al-4V and stainless-steel screws was significantly higher than (1.6-3.4 times) that of the control screws. The specific activity of alkaline phosphatase in extracts of tissue from around the implanted screws peaked 6 days after insertion, with significantly higher values at 5, 6, and 7 days postoperatively for the heat-treated screws than for the controls. The extent of calcification also was higher at all time intervals for the heat-treated screws than for the controls. The histological evaluation of formation of bone between the ridges of the implanted screw corroborated the mechanical and biochemical measurements. At each time interval, a more mature bone was noted around the heat-treated screws than around the controls. It was concluded that the heat treatment of metal implants before insertion can result in augmentation of osseous ingrowth 1.6-5.3 times that into control implants in an in vivo experimental model.
通过机械、组织学和生化方法，研究了植入大鼠股骨髓腔内的由不锈钢（316L型）和Ti-6Al-4V制成的对照（未处理）螺钉和热处理螺钉的骨生长动力学。植入后，随着时间的推移，通过螺钉的界面剪切强度反映出骨长入有渐进性且显著的增加。在植入后长达35天的所有时间间隔内，热处理的Ti-6Al-4V螺钉和不锈钢螺钉的剪切强度显著高于对照螺钉（1.6至3.4倍）。植入螺钉周围组织提取物中碱性磷酸酶的比活性在插入后6天达到峰值，术后5、6和7天，热处理螺钉的值显著高于对照螺钉。在所有时间间隔内，热处理螺钉的钙化程度也高于对照螺钉。对植入螺钉棱嵴间骨形成的组织学评估证实了机械和生化测量结果。在每个时间间隔，热处理螺钉周围的骨比对照螺钉周围的骨更成熟。得出的结论是，在体内实验模型中，金属植入物在插入前进行热处理可使骨长入增加至对照植入物的1.6至5.3倍。
