Pernthaner A, Stankiewicz M, Bisset S A, Jonas W E, Cabaj W, Pulford H D
AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
Int J Parasitol. 1995 Apr;25(4):523-9. doi: 10.1016/0020-7519(94)00149-i.
Blastogenic activity, eosinophil and total white blood cell counts (TWBC) were examined over a period of 14 weeks in Romney lambs, genetically resistant or susceptible to gastrointestinal nematodes. The lambs were infected with 5000 infective Trichostrongylus colubriformis larvae twice weekly. Compared to preinfection levels, the blastogenic activity of unstimulated lymphocytes in lambs of both lines peaked at week 3, and was significantly higher in resistant than in susceptible lambs. These changes may have been due to in vivo polyclonal activation. Lymphocytes from susceptible sheep responded more strongly to Con A, PHA and PWM than cells from resistant sheep. Counts per minute (c.p.m) for Con A- and PHA-stimulated lymphocytes increased in both lines of sheep from week 2 to week 7 and then returned to initial levels. An increase in c.p.m. in PWM-stimulated cell cultures was observed from weeks 3 to 5 in both groups. The blastogenic activity for LPS-stimulated cultures was significantly higher for resistant than susceptible sheep at weeks 3 and 4. No significant correlations between the decline in faecal egg counts (FEC) and the blastogenic activity was observed. Eosinophil counts in peripheral blood began to increase one week earlier in resistant than in susceptible sheep. No significant correlation between FEC and eosinophil counts was observed in resistant lambs, whereas in susceptible lambs a significant correlation was found between FEC and eosinophil counts at some sampling times. TWBC in resistant lambs steadily increased with infections whereas susceptible lambs showed a decrease until week 5 and then steadily increased. There was no significant correlation between the decline in FEC and TWBC.(ABSTRACT TRUNCATED AT 250 WORDS)
在14周的时间里，对罗姆尼羔羊进行了致有丝分裂活性、嗜酸性粒细胞和总白细胞计数（TWBC）的检测，这些羔羊在遗传上对胃肠道线虫具有抗性或易感性。每周两次给羔羊感染5000条感染性结肠毛圆线虫幼虫。与感染前水平相比，两个品系羔羊中未刺激淋巴细胞的致有丝分裂活性在第3周达到峰值，且抗性羔羊的活性显著高于易感羔羊。这些变化可能是由于体内多克隆激活所致。易感绵羊的淋巴细胞对刀豆蛋白A（Con A）、植物血凝素（PHA）和美洲商陆有丝分裂原（PWM）的反应比抗性绵羊的细胞更强。从第2周到第7周，两个品系绵羊中Con A和PHA刺激的淋巴细胞的每分钟计数（c.p.m）均增加，然后恢复到初始水平。两组在第3周和第5周均观察到PWM刺激的细胞培养物中c.p.m增加。在第3周和第4周，抗性绵羊中脂多糖（LPS）刺激培养物的致有丝分裂活性显著高于易感绵羊。未观察到粪便虫卵计数（FEC）下降与致有丝分裂活性之间存在显著相关性。抗性绵羊外周血中的嗜酸性粒细胞计数比易感绵羊提前一周开始增加。在抗性羔羊中未观察到FEC与嗜酸性粒细胞计数之间存在显著相关性，而在易感羔羊中，在某些采样时间发现FEC与嗜酸性粒细胞计数之间存在显著相关性。抗性羔羊的TWBC随着感染而稳步增加，而易感羔羊在第5周前呈下降趋势，然后稳步增加。FEC下降与TWBC之间无显著相关性。（摘要截断于250字）
