Rothwell T L, Windon R G, Horsburgh B A, Anderson B H
Department of Veterinary Pathology, University of Sydney, New South Wales, Australia.
Int J Parasitol. 1993 Apr;23(2):203-11. doi: 10.1016/0020-7519(93)90142-l.
Lambs with genetically determined increased immunological responsiveness to Trichostrongylus colubriformis (high responders) had more eosinophils in cutaneous reactions to the mitogen phytohaemagglutinin (PHA) both before and during infection compared with those bred for susceptibility (low responders). In contrast, eosinophil numbers in both blood and cutaneous reactions elicited by third-stage T. colubriformis larval antigen were similar in high and low responders before infection. Following vaccination and challenge, high responders had elevated eosinophil numbers in blood and antigen-stimulated skin. In unselected sheep, although eosinophil numbers in cutaneous reactions to PHA were related to responsiveness to a challenge infection with T. colubriformis, there was a closer relationship between blood eosinophil numbers and responsiveness. Infection with T. colubriformis increased eosinophil numbers in cutaneous reactions to PHA and appeared to augment the difference between eosinophil counts in high and low responder sheep. Measurement of the ability to produce eosinophil activating factors, or for eosinophils to respond to such factors might therefore be useful in identifying individual sheep with increased responsiveness to T. colubriformis infection.
与选育出的易感羔羊（低反应者）相比，具有遗传决定的对蛇形毛圆线虫免疫反应性增强的羔羊（高反应者），在感染前和感染期间，对丝裂原植物血凝素（PHA）的皮肤反应中嗜酸性粒细胞更多。相比之下，在感染前，高反应者和低反应者由第三期蛇形毛圆线虫幼虫抗原引起的血液和皮肤反应中的嗜酸性粒细胞数量相似。接种疫苗并受到攻击后，高反应者血液和抗原刺激皮肤中的嗜酸性粒细胞数量升高。在未经过选择的绵羊中，虽然对PHA的皮肤反应中的嗜酸性粒细胞数量与对蛇形毛圆线虫攻击感染的反应性有关，但血液嗜酸性粒细胞数量与反应性之间的关系更为密切。感染蛇形毛圆线虫会增加对PHA皮肤反应中的嗜酸性粒细胞数量，并且似乎扩大了高反应者和低反应者绵羊之间嗜酸性粒细胞计数的差异。因此，测量产生嗜酸性粒细胞激活因子的能力，或嗜酸性粒细胞对此类因子的反应能力，可能有助于识别对蛇形毛圆线虫感染反应性增强的个体绵羊。
