刚地弓形虫：通过快速酶联免疫吸附测定法检测鼠血清对速殖子和包囊抗原的反应性

Toxoplasma gondii: reactivity of murine sera against tachyzoite and cyst antigens via FAST-ELISA.

作者信息

Zhang Y W, Smith J E

机构信息

Department of Pure and Applied Biology, University of Leeds, U.K.

出版信息

Int J Parasitol. 1995 May;25(5):637-40. doi: 10.1016/0020-7519(94)00139-f.

DOI:10.1016/0020-7519(94)00139-f

PMID:7635642

Abstract

The murine serological response to Toxoplasma gondii tachyzoite and cyst antigens was determined using FAST-ELISA. The serum IgG response to tachyzoite antigen was much stronger than that to cyst antigen. Adsorption of immune sera with tachyzoite antigen sharply reduced the reactivity in ELISA with tachyzoite antigen, but had no effect on the titre against cyst antigen, implying that there is virtually no antigenic overlap between the 2 stages. In sequential sera from infected mice the IgG antibody response against tachyzoites was always higher than the response to cyst antigen, whereas the IgM response to cysts was always higher than that to tachyzoites and remained detectable for at least 11 months.

摘要

使用快速酶联免疫吸附测定法（FAST-ELISA）检测小鼠对刚地弓形虫速殖子和包囊抗原的血清学反应。血清中针对速殖子抗原的IgG反应比针对包囊抗原的反应强得多。用速殖子抗原吸附免疫血清可显著降低酶联免疫吸附测定法中对速殖子抗原的反应性，但对包囊抗原的效价没有影响，这意味着这两个阶段之间几乎没有抗原重叠。在感染小鼠的连续血清中，针对速殖子的IgG抗体反应始终高于对包囊抗原的反应，而针对包囊的IgM反应始终高于对速殖子的反应，并且至少在11个月内均可检测到。