通过快速酶联免疫吸附测定法测定针对刚地弓形虫包囊和速殖子抗原的血清学反应性。
Serological reactivity against cyst and tachyzoite antigens of Toxoplasma gondii determined by FAST-ELISA.
作者信息
Zhang Y W, Fraser A, Balfour A H, Wreghitt T G, Gray J J, Smith J E
机构信息
Department of Pure and Applied Biology, University of Leeds.
出版信息
J Clin Pathol. 1995 Oct;48(10):908-11. doi: 10.1136/jcp.48.10.908.
Abstract
AIMS
To obtain quantitative data on the human serological response to Toxoplasma gondii tachyzoite and bradyzoite antigens.
METHODS
Serum samples from 30 patients who had positive antibody titres against T gondii and from 14 who were seronegative, together with sequential serum samples from four infected individuals, were screened by FAST-ELISA.
RESULTS
Serum samples from the 30 seropositive patients showed high IgG and IgM titres against the T gondii tachyzoite antigen but very low responses to cyst antigen. This result was borne out in sequential serum samples from patients with toxoplasmosis.
CONCLUSION
Antibody recognition of the cystic stage of T gondii is low, implying that either this stage is poorly immunogenic or that the antigen load is low.
摘要
目的
获取人类对刚地弓形虫速殖子和缓殖子抗原血清学反应的定量数据。
方法
采用快速酶联免疫吸附测定法（FAST-ELISA）对30例抗弓形虫抗体滴度呈阳性的患者以及14例血清学阴性患者的血清样本，连同4例感染个体的系列血清样本进行检测。
结果
30例血清学阳性患者的血清样本显示，其针对弓形虫速殖子抗原的IgG和IgM滴度较高，但对包囊抗原的反应极低。弓形虫病患者的系列血清样本也证实了这一结果。
结论
对弓形虫包囊期的抗体识别率较低，这意味着该阶段免疫原性较差或抗原负荷较低。
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