Espada J, Bertolesi G E, Trigoso C I, Gamallo C, Stockert J C
Department of Pathology, La Paz Hospital, Faculty of Medicine, Autonomous University of Madrid, Spain.
Histochem J. 1995 Apr;27(4):318-22. doi: 10.1007/BF00398974.
The N-quaternized derivative of dimethyl-POPOP (termed Q4) induces a bluish-green fluorescent reaction in mast cell granules from paraffin sections and cell smears, in addition to a previously described bluish-white fluorescent reaction in chromatin DNA. The chromatin reaction was abolished by staining the samples either with Mayer's Haematoxylin before Q4 treatment or by Q4 treatment at pH 1.5. The reaction in mast cell granules was absent after substrate methylation. The staining sequence Haematoxylin-Eosin-Q4 also worked well in paraffin sections, allowing the observation of the current histological image under bright-field illumination as well as double-colour emission under fluorescence microscopy. The sequence is proposed as a new diagnostic procedure for demonstrating mast cell granules.
二甲基-POPOP的N-季铵化衍生物（称为Q4）除了能在染色质DNA中引发先前描述的蓝白色荧光反应外，还能在石蜡切片和细胞涂片的肥大细胞颗粒中引发蓝绿色荧光反应。通过在Q4处理前用梅耶苏木精对样品进行染色，或在pH 1.5条件下进行Q4处理，可消除染色质反应。底物甲基化后，肥大细胞颗粒中的反应消失。苏木精-伊红-Q4染色顺序在石蜡切片中也效果良好，既可以在明场照明下观察当前的组织学图像，也可以在荧光显微镜下观察双色发射。该顺序被提议作为一种显示肥大细胞颗粒的新诊断程序。
