默克尔细胞癌细胞系的辐射敏感性。

Radiation sensitivity of Merkel cell carcinoma cell lines.

作者信息

Leonard J H, Ramsay J R, Kearsley J H, Birrell G W

机构信息

Queensland Radium Institute Laboratory, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.

出版信息

Int J Radiat Oncol Biol Phys. 1995 Jul 30;32(5):1401-7. doi: 10.1016/0360-3016(94)00610-W.

DOI:10.1016/0360-3016(94)00610-W

PMID:7635780

Abstract

PURPOSE

Merkel cell carcinoma (MCC), being a small cell carcinoma, would be expected to be sensitive to radiation. Clinical analysis of patients at our center, especially those with macroscopic disease, would suggest the response is quite variable. We have recently established a number of MCC cell lines from patients prior to radiotherapy, and for the first time are in a position to determine their sensitivity under controlled conditions.

METHODS AND MATERIALS

Some of the MCC lines grew as suspension cultures and could not be single cell cloned; therefore, it was not possible to use clonogenic survival for all cell lines. A tetrazolium based (MTT) assay was used for these lines, to estimate cell growth after gamma irradiation. Control experiments were conducted on lymphoblastoid cell lines (LCL) and the adherent MCC line, MCC13, to demonstrate that the two assays were comparable under the conditions used.

RESULTS

We have examined cell lines from MCC, small cell lung cancer (SCLC), malignant melanomas, Epstein Barr virus (EBV) transformed lymphocytes (LCL), and skin fibroblasts for their sensitivity to gamma irradiation using both clonogenic cell survival and MTT assays. The results show that the tumor cell lines have a range of sensitivities, with melanoma being more resistant (surviving fraction at 2 Gy (SF2) 0.57 and 0.56) than the small cell carcinoma lines, MCC (SF2 range 0.21-0.45, mean SF2 0.30, n = 8) and SCLC (SF2 0.31). Fibroblasts were the most sensitive (SF2 0.13-0.20, mean 0.16, n = 5). The MTT assay, when compared to clonogenic assay for the MCC13 adherent line and the LCL, gave comparable results under the conditions used.

CONCLUSION

Both assays gave a range of SF2 values for the MCC cell lines, suggesting that these cancers would give a heterogeneous response in vivo. The results with the two derivative clones of MCC14 (SF2 for MCC14/1 0.38, MCC14/2 0.45) would further suggest that some of them may develop resistance during clonogenic evolution.

摘要

目的

默克尔细胞癌（MCC）作为一种小细胞癌，预计对放疗敏感。我们中心对患者的临床分析，尤其是那些有肉眼可见病灶的患者，表明其反应差异很大。我们最近从放疗前的患者中建立了多个MCC细胞系，首次能够在可控条件下确定它们的敏感性。

方法和材料

一些MCC细胞系以悬浮培养方式生长，无法进行单细胞克隆；因此，不可能对所有细胞系都采用克隆形成存活法。对于这些细胞系，使用基于四唑盐的（MTT）测定法来估计γ射线照射后的细胞生长情况。对淋巴母细胞系（LCL）和贴壁的MCC细胞系MCC13进行了对照实验，以证明在所用条件下这两种测定方法具有可比性。

结果

我们使用克隆形成细胞存活法和MTT测定法，检测了来自MCC、小细胞肺癌（SCLC）、恶性黑色素瘤、爱泼斯坦 - 巴尔病毒（EBV）转化淋巴细胞（LCL）和皮肤成纤维细胞的细胞系对γ射线照射的敏感性。结果表明，肿瘤细胞系具有不同的敏感性，黑色素瘤比小细胞癌系更具抗性（2 Gy时的存活分数（SF2）为0.57和0.56），MCC（SF2范围为0.21 - 0.45，平均SF2为0.30，n = 8）和SCLC（SF2为0.31）。成纤维细胞最敏感（SF2为0.13 - 0.20，平均为0.16，n = 5）。在所用条件下，与MCC13贴壁细胞系和LCL的克隆形成测定法相比，MTT测定法给出了可比的结果。