集胞藻6803菌株DNA A区域的遗传结构
Genetic structure of the dnaA region of the cyanobacterium Synechocystis sp. strain PCC6803.
作者信息
Richter S, Messer W
机构信息
Max-Planck-Institut für molekulare Genetik, Berlin-Dahlem, Germany.
出版信息
J Bacteriol. 1995 Aug;177(15):4245-51. doi: 10.1128/jb.177.15.4245-4251.1995.
Abstract
We have cloned and sequenced the dnaA region of Synechocystis sp. strain PCC6803, a bacterium with a light-dependent cell cycle. The dnaA gene product, DnaA, is the central factor for replication initiation in bacteria. The deduced amino acid sequence of the protein encoded by the cyanobacterial dnaA gene is 45% identical to DnaA of Bacillus subtilis and fits very well into the homology pattern of the known eubacterial DnaA proteins. The genetic environment of the Synechocystis sp. strain PCC6803 dnaA gene is completely different from the one in other eubacteria. An open reading frame of unknown function, orf134, was detected upstream of dnaA. The purT gene homolog encoding the glycinamide ribonucleotide transformylase T starts about 200 bp away from this open reading frame in the opposite direction. Downstream of the dnaA gene we detected the start of the psbDC operon, which codes for the photosystem II reaction center proteins D2 and CP43 that are involved in the positioning of chlorophyll a.
摘要
我们已经克隆并测序了集胞藻属PCC6803菌株的dnaA区域，该细菌具有光依赖型细胞周期。dnaA基因产物DnaA是细菌复制起始的核心因子。蓝藻dnaA基因编码的蛋白质推导氨基酸序列与枯草芽孢杆菌的DnaA有45%的同源性，并且与已知真细菌DnaA蛋白的同源模式非常吻合。集胞藻属PCC6803菌株dnaA基因的遗传环境与其他真细菌完全不同。在dnaA上游检测到一个功能未知的开放阅读框orf134。编码甘氨酰胺核苷酸转甲酰基酶T的purT基因同源物在相反方向上距离这个开放阅读框约200 bp处起始。在dnaA基因下游，我们检测到psbDC操纵子的起始，该操纵子编码参与叶绿素a定位的光系统II反应中心蛋白D2和CP43。
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